References

Reviews on basic technologies

Frenzel, A., Schirrmann, T. and Hust, M. (2016). Phage display-derived human antibodies in clinical development and therapy. mAbs 8: 1177-1194 (PubMed)

Frenzel, A., Kügler, J., Wilke, S., Schirrmann, T. & Hust, M. (2014). Construction of human antibody gene libraries and selection of antibodies by phage display. Methods Molecular Biology 1060: 215-243 (PubMed)

Kügler, J., Zantow, J., Meyer, T., & Hust, M. (2013). Oligopeptide M13 Phage Display in Pathogen Research. Viruses 5:2531-2545 (PubMed)

Bradbury, A., Sidhu, S., Dübel, S. & McCafferty, J. (2011). Beyond natural antibodies: the power of in vitro display technologies. Nature Biotechnology 29, 245-254 (PubMed)

Schirrmann, T., Meyer, T., Schütte, M., Frenzel, A., & Hust, M. (2011). Phage display for the generation of antibodies for proteome research, diagnostics and therapy. Molecules 16, 412-426 (PubMed)

Dübel, S., Stoevesandt, O., Taussig, M.J. & Hust, M. (2010). Generating recombinant antibodies to the complete human proteome. Trends in Biotechnology 28, 333-339 (PubMed)

Dübel, S. (2007) Recombinant therapeutic antibodies. Applied Microbiology and Biotechnology 74, 723-729 (PubMed)

Hust, M. & Dübel, S. (2004). Mating antibody phage display to proteomics. Trends in Biotechnology 22, 8-14 (PubMed)

 

Research articles about success stories from YUMAB technologies

Antibody discovery and antibody engineering

Zehner, M., Marschall, A.L., Bos, E., Schloetel, J.G., Kreer, C., Fehrenschild, D., Limmer, A., Ossendorp, F., Lang, T., Koster, A.J., Dübel, S. and Burgdorf S. (2015). The translocon protein sec61 mediates antigen transport from endosomes in the cytosol for cross-presentation to CD8(+) T cells. Immunity 42: 850-863 (PubMed)

Postel, S., Deredge, D., Bonsor, D.A., Yu, X., Diederichs, K., Helmsing, S., Vromen, A., Friedler, A., Hust, M., Egelman, E.H., Beckett, D., Wintrode, P.L., and Sundberg, E.J. (2016). Bacterial flagellar capping proteins adopt diverse oligomeric states. eLIFE 5:e18857 (PubMed)

Derman, Y., Selby, K., Miethe, S., Frenzel, A., Liu, Y., Rasetti-Escargueil, C., Avril, A., Pelat, T., Urbain, R., Fontayne, A., Thullier, P., Sesardic, D., Lindström, M., Hust, M. and Korkeala, H. (2016). Neutralization of Botulinum Neurotoxin Type E by a Humanized Antibody. Toxins 8: 257 (PubMed)

Miethe, S., Mazuet, C., Liu, Y., Tierney, R., Rasetti-Escargueil, C., Avril, A., Frenzel, A., Thullier, P., Pelat, T., Urbain, R., Fontayne, A., Sesardic, D., Hust, M. and Popoff, M.-R. (2016). Development of Germline-Humanized Antibodies Neutralizing Botulinum Neurotoxin A and B. PLoS ONE 11: e0161446 (PubMed)

Schieferdecker, A., Oberle, A., Thiele, B., Hofmann, F., Göthel, M, Miethe, S., Hust, M., Braig, F., Voigt, M., Koch-Nolte, F., Haag, F., Alawi, M., Indenbirken, D., Grundhoff, A., Bokemeyer, C., Bacher, U., Kröger, N. and Binder, M. (2016). A transplant immunome deep sequencing and screening platform defines a unique targetable epitope fingerprint of multiple myeloma. Blood 127: 3202-3214 (PubMed)

Kibat, J., Schirrmann, T., Knape, M.J., Helmsing, S., Meier, D., Hust, M., Schröder, C., Bertinetti, D., Pardes, K., Funk, M., Lages, A., Giese, N., Herberg, F. W., Dübel, S. and Hoheisel, J.D.  (2016). Utilisation of antibody microarrays for the selection of specific and informative antibodies from recombinant library binders of unknown quality. New Biotechnology 33: 574-581 (PubMed)

Wirsing, L., Klawonn, F., Sassen, W., Lünsdorf, H., Probst, C., Hust, M., Mendel, R., Kruse, T. & Jänsch, L. (2015). Linear discriminant analysis identifies mitochondrially localized proteins in Neurospora crassa. Journal of Proteome Research 14: 3900-3911 (Pubmed)

Miethe, S., Rasetti-Escargueil, C., Avril, A., Liu, Y., Chahboun, S., Korkeala, H., Mazuet, C., Popoff, M.-R., Pelat, T., Thullier. P., Sesardic, D. & Hust, M. (2015). Development of human-like scFv-Fc neutralizing botulinum neurotoxin E. PLoS ONE 10: e0139905 (Pubmed)

Kügler, J., Wilke, S. Meier, D., Tomszak, F.,  Frenzel, A. , Schirrmann, T., Dübel, S.,  Garritsen, H.,  Hock, B., Toleikis, L., Schütte, M. & Hust, M. (2015). Generation and analysis of the improved human HAL9/10 antibody phage display libraries. BMC-Biotechnology 15: 10 (PubMed)

Fuchs, M., Kämpfer, S., Helmsing, S., Spallek, R., Oehlmann, W., Prilop, W., Frank, R., Dübel, S., Mahavir, S. and Hust, M. (2014). Novel human recombinant antibodies against Mycobacterium tuberculosis antigen 85B. BMC-Biotechnology 14: 68 (PubMed)

Trott, M., Weiß, S., Antoni, S., Koch, J., von Briesen, H., Hust, M. and Dietrich, U. (2014). Functional characterization of two scFv-Fc antibodies from an HIV controller selected on soluble HIV-1 Env complexes: a neutralizing V3- and a trimer-specific gp41 antibody. PLoS ONE 9: e97478 (PubMed)

Hülseweh, B., Rülker, T., Pelat, T., Langermann, C., Frenzel, A., Schirrmann, T., Dübel, S., Thullier, P. & Hust, M. (2014). Human-like antibodies neutralizing Western equine encephalitis virus. mAbs 6: 717-726 (PubMed)

Miethe, S., Rasetti-Escargueil, C., Liu, Y., Chahboun, S., Pelat, T., Avril, A., Frenzel, A., Schirrmann, T., Thullier, P., Sesardic, D. & Hust, M. (2014). Development of neutralizing scFv-Fc against botulinum neurotoxin A light chain from a macaque immune library. mAbs 6: 446-459 (PubMed)

Jäger, V., Büssow, K., Wagner, A., Weber, S., Hust, M., Frenzel, A. & Schirrmann, T. (2013). High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells. BMC-Biotechnology 13:52 (PubMed)

Wezler, X., Hust, M., Helmsing, S., Schirrmann, T. & Dübel, S. (2012). Human antibodies targeting CD30+ lymphomas. Human Antibodies 21, 13-28 (PubMed)

Rülker, T., Voß, L., Thullier, P., O’Brien, L.M., Pelat, T., Perkins, S.D., Langemann, C., Schirrmann, T., Dübel, S., Marschall, H.-J., Hust, M. & Hülseweh, B. (2012). Isolation and characterisation of a human-like antibody fragment (scFv) that inactivates VEEV in vitro and in vivo. PLoS ONE 7: e37242 (PubMed)

Zhang, C., Helmsing, S., Zagrebelsky, M., Schirrmann, T., Marschall, A.L.J., Schüngel, M., Korte, M., Hust, M. & Dübel, S. (2012). Suppression of p75 Neurotrophin Receptor Surface Expression with Intrabodies Influences Bcl-xL mRNA Expression and Neurite Outgrowth in PC12 Cells. PLoS ONE 7: e30684 (PubMed)

Colwill, K., Renewable Protein Binder Working Group & Gräslund, S. (2011). A roadmap to generate renewable protein binders to the human proteome. Nature Methods 8, 551–558 (PubMed)

Hust, M., Meyer, T., Voedisch, B., Rülker, T., Thie, H., Schütte, M., Helmsing, S., Meier, D., Schirrmann, T. & Dübel, S. (2011). A human scFv antibody generation pipeline for proteome research, Journal of Biotechnology 152,159-170 (PubMed)

Meyer, T., Stratmann-Selke, J., Meens, J., Schirrmann, T., Gerlach, G.F., Frank, R., Dübel, S., Strutzberg-Minder, K.& Hust, M. (2010). Isolation of OmpD specific scFv fragments for the diagnosis of Salmonella Typhimurium. Veterinary Microbiology 147, 162-169 (PubMed)

Thie, H., Toleikis, L., Li, J., von Wasielewski, R., Bastert, G., Schirrmann, T., Esteves, I. T., Behrens, C. K., Fournes, B., Fournier, N., de Romeuf, C., Hust, M. and Dübel, S. (2011). Rise and fall of an anti-MUC1 specific antibody. PLoS ONE 6: e15921 (PubMed)

Mersmann, M., Meier, D., Mersmann, J., Helmsing, S., Nilsson, P., Gräslund, S., Structural Genomics Consortium, Colwill, K., Hust M. & Dübel, S. (2010). Towards Proteome Scale Antibody Selections using Phage Display. New Biotechnology 27, 118-128 (PubMed)

Schütte, M., Thullier, P., Pelat, T., Wezler, X., Rosenstock, P., Dominik, Hinz, D., Kirsch, M.I., Hasenberg, M., Frank, R., Schirrmann, T., Gunzer, M., Hust, M. & Dübel, S. (2009). Identification of a putative Crf splice variant and generation of recombinant antibodies for the specific detection of Aspergillus fumigatus. PLoS ONE 4:e6625 (PubMed)

Pelat, T., Hust, M., Hale, M., Lefranc, M.-P., Dübel, S. & Thullier, P. (2009). Isolation of a human-like antibody fragment (scFv) that neutralizes ricin biological activity. BMC-Biotechnology 8:66 (PubMed)

Kirsch, M.I., Hülseweh, B., Nacke, C., Rülker, T., Schirrmann, T., Marschall, H.-J., Hust, M. & Dübel, S. (2008). Development of human antibody fragments using antibody phage display for the detection and diagnosis of Venezuelan equine encephalitis virus (VEEV). BMC-Biotechnology 8:66 (PubMed)

Pelat, T., Hust, M., Laffly. E., Condemine, F., Bottex, C., Vidal, D., Lefranc, M.P., Dübel, S. & Thullier, P. (2007). A high affinity, human like antibody fragment (scFv) neutralising lethal factor (LF) of Bacillus anthracis by inhibiting the PA-LF complex formation. Antimicrobial agents and chemotherapy 51, 2758-2764 (PubMed)

Target antigen discovery

Zantow, J., Just, S., Lagkouvardos, I., Kisling, S., Dübel, S., Lepage, P., Clavel, T. and Hust, M. (2016). Mining gut microbiome oligopeptides by functional metaproteome display. Scientific Reports 6:34337 (PubMed)

Becker, M., Felsberger, A., Frenzel, A., Shattuck, W.M.C, Dyer, M., Kügler, J., Zantow, J., Mather, T., & Hust, M. (2015). Application of M13 Phage Display for Identifying Immunogenic Proteins from Tick (Ixodes scapularis) Saliva. BMC Biotechnology 15: 43 (PubMed)

Zhou, M., Meyer, T., Koch, S., Koch, J., Brill, B., von Briesen, H., Benito, J.M., Soriano, V., Haberl, A., Bickel, M., Dübel, S., Hust, M. & Dietrich, U. (2013). Identification of an epitope in the membrane proximal external region of gp41 targeted by neutralizing antibodies in plasma from an elite controller using an Env-tailored phage display library. European Journal of Immunology 43: 499-509 (PubMed)

Meyer, T., Schirrmann, T., Frenzel, A., Miethe, S., Stratmann-Selke, J., Gerlach, G.F., Strutzberg-Minder, K., Dübel, S. & Hust, M. (2012). Identification of immunogenic proteins and generation of antibodies against Salmonella Typhimurium using phage display. BMC Biotechnology 12:29 (PubMed)

Naseem, S., Meens, J., Jores, J., Heller, M., Dübel, S., Hust, M. & Gerlach, G.F. (2010). Phage display-based identification and potential diagnostic application of novel antigens from Mycoplasma mycoides subsp. mycoides small colony type. Veterinary Microbiology 142, 285-292 (PubMed)

Kügler, J., Nieswandt, S., Gerlach, G.F., Meens, J., Schirrmann, T. & Hust, M. (2008). Identification of immunogenic polypeptides from a Mycoplasma hyopneumoniae genome library by phage display. Applied Microbiology and Biotechnology 80, 447- 458 (PubMed)

Hust, M., Meysing, M, Schirrmann, T., Selke, M., Meens, J., Gerlach, G.-F. & Dübel, S. (2006). Enrichment of open reading frames presented on bacteriophage M13 using Hyperphage. Biotechniques 41, 335-342 (PubMed)